Title : Amplified fragment length polymorphism of clinical and 1 environmental Vibrio cholerae from a freshwater 2 environment in a cholera - endemic area , India
نویسندگان
چکیده
Arti Mishra 1 *, Neelam Taneja 1§* , Ram K Sharma 2 *, Rahul Kumar 2 *, Naresh C Sharma 3 *, 4 Meera Sharma 1 *. 5 1 Department of Medical Microbiology. Post Graduate Institute of Medical Education and 6 Research, Chandigarh, India-160012. 7 2 Biotechnology Division. Institute of Himalayan Bioresource and Technology , Palampur, 8 Himachal Pradesh, India176061. 9 3 Laboratory Department, Maharishi Valmiki Infectious Diseases Hospital, Kingsway Camp, 10 Delhi 110009, India. 11 *These authors contributed equally to this work 12 § Corresponding author 13 Email addresses: 14 AM: [email protected] 15 NT: [email protected] 16 RKS: [email protected] 17 RK: [email protected] 18 NCS: [email protected] 19 MS: [email protected] 20
منابع مشابه
Amplified fragment length polymorphism of clinical and environmental Vibrio cholerae from a freshwater environment in a cholera-endemic area, India
BACKGROUND The region around Chandigarh in India has witnessed a resurgence of cholera. However, isolation of V. cholerae O1 from the environment is infrequent. Therefore, to study whether environmental nonO1-nonO139 isolates, which are native to the aquatic ecosystem, act as precursors for pathogenic O1 strains, their virulence potential and evolutionary relatedness was checked. METHODS V. c...
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Vibrio cholerae, the causative agent of major epidemics of diarrheal disease in Bangladesh, South America, Southeastern Asia, and Africa, was isolated from clinical samples and from aquatic environments during and between epidemics over the past 20 years. To determine the evolutionary relationships and molecular diversity of these strains, in order to understand sources, origin, and epidemiolog...
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Cholera outbreak is more common in developing countries. The causative agent of the disease is Vibrio cholerae strains O1 and O139. Traditional diagnostic testing for Vibrio is not always reliable, because Vibrio can enter a viable but non cultivable state. Therefore, nucleic acid-based tests have emerged as a useful alternative to traditional enrichment testing. In this investigation, a...
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Incidence of epidemic Vibrio cholerae serogroup O139 has declined in cholera endemic countries. However, sporadic cholera caused by V. cholerae O139 with notable genetic changes is still reported from many regions. In the present study, 42 V. cholerae O139 strains isolated from 2001 to 2006 in Delhi, India, were retrospectively analyzed to understand their phenotype and molecular characteristic...
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Background: The virulence of a pathogenic Vibrio cholerae is dependent on a discrete set of genetic determinants. In this study, we determined the distribution of virulence determinants among the clinical and environmental isolates of V. cholerae. Methods: The antibiotic resistance profiles of the isolates were determined using standard disk diffusion assay. PCR assay was performed to analyze t...
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